mouse anti mys  (Developmental Studies Hybridoma Bank)

Journal: Nature Communications

Article Title: Ecdysone signaling-induced dumpless1 expression controls nurse cell dumping in Drosophila oogenesis

doi: 10.1038/s41467-025-63973-3

Figure Lengend Snippet: a , b Barplots of the KEGG pathways enriched for the DEGs in the ovaries between Gal80 ts ; c329b> and Gal80 ts ; c329b > EcR DN ( a ) and between w 1118 and dumpless1 -/- mutant females ( b ), respectively. The red asterisk indicates the shared KEGG pathways. c A Venn diagram showing the overlapping genes in the ECM-receptor interaction pathway enriched in both RNA-Seq datasets. d Differences in the expression of ECM-receptor integrin βPS in the ovaries of two pairs of comparison. e – n ’ Integrin βPS expression (green) in the egg chambers at stages 9–12, detected by anti-integrin βPS antibody, from w 1118 ( e - e ’, f - f ’, i - i ’ and l - l ’), dumpless1 -/- ( g - g’ , j - j ’ and m - m ’) and dumpless1 ∆ZAD ( h - h ’, k - k ’ and n - n ’) mutant. o Quantified fluorescence intensity of integrin βPS in the plasma membrane of SFCs at stages 9–12 of w 1118 (From left to right: n = 6, 14, 14, 13, respectively). Different lowercase letters represent statistical significance by one-way ANOVA. p Quantified fluorescence intensity of integrin βPS in the plasma membrane of SFCs at stages 10–12 of w 1118 , dumpless1 -/- and dumpless1 ∆ZAD mutants. From left to right: n = 14, 15, 12, 14, 16, 14, 13, 12, 14, respectively. Two-way ANOVA was made for multiple comparisons. q - r ’ Integrin βPS expression (green) in egg chambers at stage 11 from the control ( Gal80 ts ; c329b > ) and ecdysone-disrupted ovaries ( Gal80 ts ; c329b > EcR DN ). s Quantification of fluorescence intensity of integrin βPS in the plasma membrane of SFCs at stage 11 of the control ( n = 6) and ecdysone-disrupted ovaries ( n = 8). Two-tailed t- test was performed. F-actin is marked by phalloidin in magenta. The white arrow indicates the plasma membrane of SFCs. Nuclei are marked by DAPI in cyan. The scale bar represents 50 µm. NC nurse cell, SFC stretch follicle cell, MFC mainbody follicle cell, OC oocyte. Data are presented as mean values ± SD.

Article Snippet: The following primary antibodies were used: anti-CG12942 antibody (1:2000, lab-made), anti-integrin βPS antibody (1:100, CF.6G11, Developmental Studies Hybridoma Bank (DSHB)), anti-Rho1 antibody (1:50, P1D9, DSHB), anti-p-MLC antibody (recognize Ser 21 p-MLC in Drosophila ) (1:50, 3671S, Cell Signaling Technology), anti-EcR antibody (1:30, Ag10.2, DSHB), anti-EcRB1 antibody (1:50, AD4.4, DSHB) and anti-β-galactosidase (1:200, Z3781, Promega),anti-GFP antibody (1:200, YM3009, Immunoway).

Techniques: Mutagenesis, RNA Sequencing, Expressing, Comparison, Fluorescence, Clinical Proteomics, Membrane, Control, Two Tailed Test

Journal: Nature Communications

Article Title: Ecdysone signaling-induced dumpless1 expression controls nurse cell dumping in Drosophila oogenesis

doi: 10.1038/s41467-025-63973-3

Figure Lengend Snippet: a – d ’ Changes of p-MLC (red) enrichment in the NCs at stages 10–11 after overexpression of integrin βPS (green) in SFCs. e , f Quantified fluorescence intensity of integrin βPS in the plasma membrane of SFCs and p-MLC in the NC cortex at stages 10-11 of Gal80 ts ; c329b> and Gal80 ts ; c329b>mys lines, respectively. e From left to right: n = 12, 13, 13, 14, respectively. f From left to right: n = 15, 13, 14, 11, respectively. g – l ’ Changes of p-MLC (red) enrichment in NCs at stages 10–12 after knockdown of integrin βPS (green) in SFCs of dumpless1 -/- mutants. m , n Quantified fluorescence intensity of integrin βPS in the plasma membrane of SFCs and p-MLC in the NC cortex at stages 10–12 of dumpless1 -/- ; Gal80 ts ; c329b >and dumpless1 -/- ; Gal80 ts ; c329b>mys RNAi line , respectively. m From left to right: n = 6, 6, 11, 14, 13, 10, respectively. n From left to right: n = 8, 6, 11, 14, 13, 10, respectively. o Knockdown of integrin βPS in SFCs partially rescues the egg length defects in dumpless1 -/- mutants ( n = 52). Two-tailed t- test was performed. The yellow arrows indicate the cortex of NCs. The scale bar represents 50 µm. NC nurse cell, SFC stretch follicle cell, MFC mainbody follicle cell, OC oocyte. Two-tailed t- test was performed. Data are presented as mean values ± SD.

Article Snippet: The following primary antibodies were used: anti-CG12942 antibody (1:2000, lab-made), anti-integrin βPS antibody (1:100, CF.6G11, Developmental Studies Hybridoma Bank (DSHB)), anti-Rho1 antibody (1:50, P1D9, DSHB), anti-p-MLC antibody (recognize Ser 21 p-MLC in Drosophila ) (1:50, 3671S, Cell Signaling Technology), anti-EcR antibody (1:30, Ag10.2, DSHB), anti-EcRB1 antibody (1:50, AD4.4, DSHB) and anti-β-galactosidase (1:200, Z3781, Promega),anti-GFP antibody (1:200, YM3009, Immunoway).

Techniques: Over Expression, Fluorescence, Clinical Proteomics, Membrane, Knockdown, Two Tailed Test

Journal: Nature Communications

Article Title: Ecdysone signaling-induced dumpless1 expression controls nurse cell dumping in Drosophila oogenesis

doi: 10.1038/s41467-025-63973-3

Figure Lengend Snippet: In responding to MFC-synthesized 20E stimulation, dumpless1 in SFCs suppresses ECM-receptor integrin βPS expression, which in turn activates Rho1-dependent cortical p-MLC enrichment to initiate actomyosin contraction. Additionally, dumpless1 also facilitates actin cables organization in NCs, thus contributing to NC dumping.

Article Snippet: The following primary antibodies were used: anti-CG12942 antibody (1:2000, lab-made), anti-integrin βPS antibody (1:100, CF.6G11, Developmental Studies Hybridoma Bank (DSHB)), anti-Rho1 antibody (1:50, P1D9, DSHB), anti-p-MLC antibody (recognize Ser 21 p-MLC in Drosophila ) (1:50, 3671S, Cell Signaling Technology), anti-EcR antibody (1:30, Ag10.2, DSHB), anti-EcRB1 antibody (1:50, AD4.4, DSHB) and anti-β-galactosidase (1:200, Z3781, Promega),anti-GFP antibody (1:200, YM3009, Immunoway).

Techniques: Synthesized, Expressing